关键词: 镉, 神经干细胞, 胶质细胞, 分化

Abstract: OBJECTIVE: To investigate the effects of cadmium chloride on differentiation of mouse neural stem cells (mNSCs) in vitro. METHODS：Primary culture of mNSCs was used as research model. mNSCs were exposed to cadmium chloride (0，0.1，0.3，1.0，3.0 and 10.0 μmol/L). MTT assay was used to access cytotoxicity at 24 and 48 h，the differentiation of mNSCs after 48 h exposure of cadmium chloride was determined by immunocytochemistry staining. RESULTS：With the increase of cadmium chloride dose，cytotoxicity on mNSCs was accentuated in a dose-dependent manner (24 h，r＝－0.91；48 h，r＝－0.96；P＜0.05). The cell viability at 10.0 μmol/L after 24 hours and at 1.0，3.0 and 10.0 μmol/L after 48 h were lower than the control (P＜0.05 or P＜0.01). Cadmium chloride influenced differentiation of mNSCs. 10.0 μmol/L significantly decreased the rate of Sox2-positive cells (P＜0.05) after 48 h exposure to cadmium chloride. The GFAP-positive also decreased in a dose-dependent manner (r＝－0.99，P＜0.05). CONCLUSION：Cadmium chloride could inhibit the differentiation of mNSCs to astrocytes.

Key words: cadmium, neural stem cells, glial cells, differentiation